| you are: Definition of quality and naturalnosti honey
Definition of reducing Sugars and sucrose. The essence of the given method consists that define optical density of a solution ferritsianida (red blood salt), honey reacting with reducing sugars. This test method includes definition of Sugars of honey before inversion.
Before test prepare a solution of red blood salt, for what 10 g zhelezosinerodistogo kalija (red blood salt) dissolve the distilled water in a flask in capacity of 1000 ml. Water add to a label. Then prepare a methyl orange solution for what 0,02 g the orange methyl dissolve in 10 ml of the hot distilled water and after cooling a solution filter. The standard solution of inverse sugar is necessary for carrying out of tests also. In these purposes take 0,381 g preliminary, within three days dried up in eksikatore sucrose or refined sugar. Them place in a measured flask in capacity 200 g and dilute with the distilled water so that the solution total was no more than 100 ml. Then in a solution add 5 ml of the concentrated hydrochloric acid, lower in a flask the thermometer and a flask with a solution place in a water bath, nagretuju to 80-82°С. A solution in a flask heat up to 67-70°С and at this temperature maintain 5 minutes Then a flask with a solution quickly cool to 20°С, add in a solution a drop of a methyl orange solution, will neutralise 25%-s' solution of alkali, add the distilled water to a label (to 200) and carefully mix.
|
